Coding

Part:BBa_K187417:Design

Designed by: Julia Pon   Group: iGEM09_Alberta   (2009-10-21)


trpA in pBA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 10
    Illegal PstI site found at 823
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 823
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 10
    Illegal PstI site found at 823
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 10
    Illegal PstI site found at 823
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Note that the gene in this plasmid starts at the start codon and ends at the stop codon. pBA does not include a promoter or terminator. pBA does include an RBS consensus sequence positioned 8 bp upstream of the ATG. We recommend that to express this gene, you use the Biobytes assembly method together with the promoter and terminator parts we have submited in pAB and pBA to assemble promoters and terminators onto the gene. As there is a range of promoters to chose from, this allows rapid manipulation of gene expression level. Using the Biobytes method, several DNA segments can be combined in just 20min per segment. See RFC 47.


Source

MG1655 E.coli genomic DNA

References